Preparation of base-deuterated 2'-deoxyadenosine nucleosides and their site-specific incorporation into DNA.
نویسندگان
چکیده
We report a four-step synthesis of 2'-deoxy-2-deuteroadenosine from 2'-deoxyadenosine in 38% overall yield. The more accessible 2'-deoxy-8-deuteroadenosine was also prepared and incorporated into DNA by automated solid phase synthesis (80% deuterium) using N6-benzoyl-2'-deoxy-8-deuteroadenosine-3'-O-(2-cyanoethyl-N,N-diisopropylphosphoramidite) in combination with acetyl-protected deoxycytidine and phenoxyacetyl-protected purine phosphoramidites.
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عنوان ژورنال:
- Nucleosides, nucleotides & nucleic acids
دوره 20 12 شماره
صفحات -
تاریخ انتشار 2001